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KMID : 0525720140190020100
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Journal of Chitin and Chitosan
2014 Volume.19 No. 2 p.100 ~ p.106
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Isolation and Characterization of Bioactive Substances from Microalgae
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Jung Sun-Hee
Park Jae-Kweon
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Abstract
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The purpose of this study is to investigate the biological properties of exopolysaccharide (EPS) isolated from microalgae. Amount of EPS extracted from the microalgae was slightly increased as time dependent manner for the extraction. However total amount of lipid extracted after EPS extraction was reversely decreased. These results suggest that alkaline condition for EPS extraction may affect on the lipid content. Optimum temperatures for carbohydrate extraction toward three different microalga named as PBRI-1, Dunaliella tertiolecta, and Botryococcus braunii were determined to be 4, 24 and 50°C respectively. Furthermore, lipid extraction on temperature dependence for EPS has similar tendency with of its EPS extraction. Meanwhile EPSs obtained from microalga did not have significant antioxidant activity, however possess significant inhibitory effect on enzyme activity of tyrosinase. Especially EPS from D. tertiolecta has about 90% of inhibitory effect on the same enzyme. These results demonstrated that EPSs from microalgae is a potential substance as for cosmetic product with having whitening activity
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KEYWORD
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Microalgae, Exopolysaccharide, Lipid, Antioxidant activity, Tyrosinase, Whitening activity
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